In sperm proteomic experiments round cells and leukocyte proteins are profiled along with\nsperm proteome. The influence of round cell and leukocyte proteins on the sperm proteome has\nnot been investigated. The objective of this study was to identify if the proteins from round cells,\nincluding leukocytes, interfere with the proteomic analysis of spermatozoa in frozen semen samples.\nProteomic profiling of sperm was performed using liquid chromatography-tandem mass spectrometry\nin four groups: Group 1 contained neat semen with round cells and leukocytes..............
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